Arthur Conan Doyle, The New Catacomb Essay Example | Topics and Well Written Essays - 250 words

Arthur Conan Doyle, The New Catacomb - Essay Example The most amazing thing about the story is its setting. The two characters are shown as sitting at Kennedy’s place, crowded with the relics of the yore, a stark contrast from the modern world that existed outside. I also found the writers delineation of the features of the characters, very interesting. To begin with, I believed that the interaction between Kennedy and Burger bordered on the good natured bantering between two friends. To me, the two friends’ journey to the new catacomb appeared to be a simple adventure. However, as the two descended into the catacomb, the details inside the catacomb, with its hidden mazes, stacked corpses and the surrounding darkness started to take a hold over me and I began to develop the premonition that something eerie is going to take place. Then the climax came with all its hidden wrath and vengeance. I was surprised and in fact awed to realize that the emotion of hurt and revenge could turn into something so treacherous and at the same time so just. The end filled me with a feeling of fear. But, deep down within me, I was to some extent satisfied that the treatment met to Kennedy by Roger was fare and well deserved, though grotesque. Overall, I found the pace of the narrative, very disciplined, terse and exciting. 2. Edgar Allan Poe, The Cask of Amontillado While reading this story I felt that the writer left many facts and details unanswered.

The Anatomy of Temperature Research Paper Example | Topics and Well Written Essays - 1250 words

The Anatomy of Temperature - Research Paper Example Temperature is an important indicator of other problems, with high temperature being indicative of fever (which usually signals an infection) and therefore can be useful in determining the health of the patient (Miller, 2009). Essential Principles Evidently, a nurse needs to be aware of how to check the temperature of a patient as it is important in so many ways. There are four major areas from which a temperature can be taken; oral, rectal, gut and skin-based (including ear and forehead). Other areas can sometimes be used, such as the vagina (Timby, 2008). The first four are the most consistent with the body temperature and therefore are the most useful in determining the true temperature of the patient. The oral temperature is the easiest of these measurements to take, although it is generally slightly lower than the ‘core’ temperature because it is influenced by outside factors such as eating and talking (Blainey, 1974). An oral thermometer can be placed below the ton gue to take this reading. The skin measurements, such as the ear and the forehead, tend to be more susceptible to changes in outside temperature and the clothing of the individual, and therefore have different ranges of what can be considered normal and healthy (Ilsley et al, 1983). Skin temperatures can easily be taken by the patient using a plastic thermometer strip, which is useful for patients who do not require hospitalization but may require some temperature monitoring (Funnell et al, 2008). This simply needs to be placed against the forehead and the approximate measurement given should be noted (Hegner et al, 2009). The gut temperature is the most difficult to take, although it is most accurate, as it involves swallowing a small thermometer. The ear has become one of the most popular ways of testing the temperature of the individual in recent years.

Knowledge And Practice Toward Breast Self Examination Nursing Essay

Knowledge And Practice Toward Breast Self Examination Nursing Essay Abstract A cross-sectional study was conducted to examine the knowledge and practice toward breast self-examination (BSE), among a sample of female nursing student in University Sultan Zainal Abidin Terengganu. Using a purposed questionnaire, a total of 40 nursing student years 2 from Nursing Department completed the questionnaire. The mean age of the respondents was 20 years (SD = 3.7). All of respondent (100%) were single. The percentages of nursing student toward breast cancer and breast self examination were high (95%) seventy seven percent (77.5%) of respondent were practice the breast self examination and only twenty two were not perform breast self examination. most of respondent are not regularly perform breast self examination, only 7.5% do it regularly. the obstacle to perform BSE is not sure how to perform BSE . The study findings suggest that the knowledge is but the practise is must be done regularly because the nurse should teach their client the right way to perform BSE. If the nurse cannot do it in right step how can they will teach their client Chapter 1 Background of study 1. Prevalence and incidence Breast cancer is the most common cancer in women in most parts of the world. There is a marked geographical variation in incidence rate.   In 2000, there were 1,050,346 cases reported with 372,969 deaths from breast cancer world-wide. The incidence ranged from an average of 95 per 100,000 in more developed countries to 20 per 100,000 in less developed countries. The incidence (number of new cancers) is steadily increasing.   The statistics are more frightening in countries like the USA where about 184,000 new cases of breast cancer are detected annually. The National Cancer Institute estimates that by age 50, one out of every 50 women will develop breast cancer. By age 80, it will rise to one in 10. If this risk is calculated over their lifetime, one in 8 women will suffer from breast cancer. One in 28 will die of the disease.   One in 3000 women develop breast cancer during pregnancy and pregnant women tend to develop them usually in their 30s. (Only 2% of breast cancers are diagnosed in pregnant women.)   Presented in another way:   Every 3 minutes, one woman is diagnosed with breast cancer (USA) Every 11 minutes, one woman dies from breast cancer (USA) Every year, 30,000 women and 200 men are diagnosed with breast cancer (UK) In countries where rates have been low, especially in Asia, the rate of increase has been the greatest with steep increases in the incidence as well as death rate (mortality).   Adapted from American Cancer Society (2003). Breast Cancer in Malaysia Breast cancer was the commonest overall cancer as well as the commonest cancer in women amongst all races from the age of 20 years in Malaysia for 2003 to 2005. Breast cancer is most common in the Chinese, followed by the Indians and then, Malays and breast cancer formed 31.1% of newly diagnosed cancer cases in women in 2003-2005. Source from the National Cancer Registry The Age Standardized Rate (ASR) of female breast cancer is 47.4 per 100,000 population (National Cancer Registry Report 2003-2005). Amongst the Chinese, it is higher at 59.9 per 100,000 population, for the Indians, the ASR is 54.2 per 100,000 and it is lowest in the Malays at 34.9 per 100,000 population. A woman in Malaysia has a 1 in 20 chance of getting breast cancer in her lifetime The cumulative life time risk of developing breast cancer for Chinese women, Indian women and Malay women were 1 in 16, 1 in 17 and 1 in 28 respectively. The peak incidence appeared to be 50-59 years old. In comparison, the next (2nd) commonest cancer in Malaysian women in 2002 2003 was cancer of the cervix, which only formed 12% and 12.9% respectively of total female cancers. The statistics for Malaysia was sourced from the National Cancer Registry Reports 2002 and 2003. Over time, the pattern of cancers, including breast cancer will be better established. It is only with continued reliable data that important decisions on planning and policy management can be made for Malaysia. Estimates by the International Agency for Research in Cancers reported that in 2000, there were 3825 cases reported and 1707 deaths from breast cancer in Malaysia. Breast cancer is the commonest female malignancy in Malaysia and all over the world. Its incidence in Malaysia in 2000 was 41.9 cases per 100,000. In 2002, 4337 cases of breast cancer were reported to the National Cancer Registry with an incidence rate of 52.8 per 100,000 and accounting for 30.4% of all diagnosed malignancies in Malaysian women. One in 9 Malaysian women has a chance of developing breast cancer. Breast cancer incidence in Malaysia is intermediate between rates of industrialized countries like the US 91/100,000 and developing countries like India 19/100,000. This situation is not permanent since the rate in developing countries is rising as women adopt lifestyles of the developed countries. Disease risk varies by ethnicity. Lifetime risk is 1 in 24 for Malays, 1 in 14 for the Chinese, and 1 in 15 for Indians. The age at diagnosis shows an opposite pattern being highest in the Indians and lowest in the Malays. The differences among ethnic groups reflect different age st ructures and lifestyle choices (parity, breast feeding practice, diet). Genetic risk factors have not been studies thoroughly in Malaysia. The risk of the disease increases with age being maximal at age 50-59. Women in Malaysia present with larger tumors and at later stages than women in the US. Certain health beliefs, lack of correct information, and inadequate health care facilities are a barrier to routine early detection and treatment of the disease Breast Cancer is easier to treat the earlier it is found. For that reason, some experts recommend that women over age 20 perform a monthly breast self examination to look for new lumps and other changes. Breast self-examination is a simple, very low cost, non-invasive adjuvant screening method for the detection of early breast cancer in women. Its purpose is important in case of a prompt reporting of breast symptoms which are important early detection messages for women of all ages, and to make women familiar with both the appearance and the feel of their breasts as early as possible. There is evidence that women who correctly practice Breast self- examination monthly are more likely to detect a lump in the early stage of its development, and early diagnosis has been reported to influence early treatment and to yield a better survival rate It was found that mortality had fallen by 31%after 6-years for women aged 40-70 at the beginning of the trial. Unfortunately despite the benefits of regular Breast self-examination, few women actually examine themselves; in fact, majority does not even know how to do Breast self examination. Although opinions conflict about the value of Breast Self Examination (BSE) 1.2 Problem statement Breast cancer is the second leading cause of cancer deaths in women today and is the most common cancer among women. .Breast cancer is the most common cancer among Malaysian women. There is a marked geographical difference in the worldwide incidence of breast cancer, with a higher incidence in developed countries compared to developing countries. According to Malaysia cancer statistic (data and figure 2006) found that the incidence of breast cancer case according to age between 0-9 years is 1 case, for to age 10-19 years 6 cases and 20-29 years is 78 cases. It is show increase in cases and it happening to all groups. Because of that, it is very important for someone to detect early sign and symptom of breast cancer. All groups should know how to perform Breast self examination Breast self-examination (BSE) is a screening method used in an attempt to detect early breast cancer. The method involves the woman herself looking at and feeling each breast for possible lumps, distortions or swelling. Breast self-examination (BSE) is a low-cost, low risk procedure that can be repeated at frequent intervals, and has been advocated as a self-performed screening procedure. 1.3 Researcher experience Trough author experience as a staff nurse in hospital and community health author found that many younger women at age 15-24 came to clinic to check the lumps because they dont know how to perform the right technique of breast self examination . Usually at this age they feel shy to expose their breast to be palpable. End of the result the condition becoming worse. And for author experience as a clinical instructor at Nursing department, a few of the student always came with complain of they have a lump at their breast but not sure it is a lump or anything else It is for this reason that the author chose to study students knowledge and practice of breast cancer examination. Having the knowledge of breast cancer could result in seeking medical attention early before complications develop. 1.4 Risk factors and symptoms of Breast cancer 1.4.1 Risk factors and sign symptoms: A risk factor is anything that increases your chance of getting a disease, For example Smoking is a risk factor for cancers of the lung, mouth, larynx, bladder, kidney, and ischemic heart diseases. But having risk factor does not mean than the disease is certain. Risk factors also can be divided into risk determinants and risk modulators. Determinants cannot be changed or influenced on the other hand risk modulators can be changed or influenced. A. Determinant risk factors: Gender: Being a woman is risk factors for breast cancer. Incidence of breast cancer in male is very low. Men account for approximately 1% of all breast cancer cases. Growing age: Incidence of breast cancer is low before 40. In absolute term advancing age is the greatest risk for developing breast cancer. About 17% of the invasive breast cancer diagnoses are women in their 40s.while, 78% of the women diagnoses the same invasive breast cancer when they are in 50s or older Genetic predisposition: Recent studies have shown that about 5% to 10% of breast cancer cases are hereditary as a result of gene changes (called mutations). The most common mutations are those of the BRCA1 and BRCA2 genes Family history of breast cancer: Research has shown that women with a family history of breast cancer have a higher risk for developing the disease. Having 1 first-degree relative (mother, sister, or daughter) with breast cancer approximately doubles a womans risk. Having 2, a first-degree relative increases her risk 5-fold. Personal history of breast cancer: A woman with cancer in one breast has a 3- to 4-fold increased risk of developing a new cancer in the other breast or in another part of the same breast. Early age at menarche and late menopause: Early menarche and late menopause both increase the risk of developing breast cancer. B. Risk modulators (Lifestyle-Related Breast Cancer Risk factors) First birth at late age and low parity: Delaying childbirth or remaining childless increase the risk of developing breast cancer. The higher parities and earlier age at first pregnancy of women in many developing countries might account for lower incidence of breast cancer in relation to developed countries. Hormone Replacement Therapy (HRT): It has become clear that long-term use (several years or more) of postmenopausal hormone therapy (PHT), particularly estrogen and progesterone combined, increases risk of breast cancer. Alcohol consumption: Recent studies have shown alcohol consumption increase the risk of breast cancer. In a summary analysis of epidemiologic studies, breast cancer risk increased between 40 and 70 percent with about two drinks daily. Obesity and high-fat diets: The relation between the obesity, high fat intake and breast cancer is complex. Most of the studies found obesity and high fat intake is the risk factors for developing breast cancer. But the relation seems to be not strong or consistent. 1.4.2 Warning symptoms of Breast cancer: Early breast cancer is usually symptom less. But there are some symptoms develop as the cancer advances. Breast lump or breast mass is the main symptoms of the breast cancer.Lump are usually painless, firm to hard and usually with irregular borders. Every lump is not cancerous, sometimes some lumps or swelling in the breast tissue may be due to hormonal changes or benign (not harmful) in nature. Beside these some others symptoms are important, like: à ¢Ã¢â€š ¬Ã‚ ¢ Lump or mass in the armpit à ¢Ã¢â€š ¬Ã‚ ¢ A change in the size or shape of the breast à ¢Ã¢â€š ¬Ã‚ ¢ Abnormal nipple discharge Usually bloody or clear-to-yellow or green fluid May look like pus (purulent) à ¢Ã¢â€š ¬Ã‚ ¢ Change in the color or feel of the skin of the breast, nipple, or areola Dimpled, puckered, or scaly Retraction, orange peel appearance Redness Accentuated veins on breast surface Change in appearance or sensation of the nipple Pulled in (retraction), enlargement, or itching à ¢Ã¢â€š ¬Ã‚ ¢ Breast pain, enlargement, or discomfort on one side only à ¢Ã¢â€š ¬Ã‚ ¢ Any breast lump, pain, tenderness, or other change in a man à ¢Ã¢â€š ¬Ã‚ ¢ Symptoms of advanced disease are bone pain, weight loss, swelling of one arm, and skin ulceration (Source: Medline plus Medical Encyclopedia: Breast Cancer.) 1.5 Research Objective 1.5.1 General Objective: To determine the level of the knowledge and practice toward breast self examination among year 2 female nursing student of University Sultan Zainal Abidin Terengganu 1.5.2 Specific objectives: 1. To determine the level of knowledge regarding breast cancer examination 2. To determine the practice of student on breast self examination. 1.5.3 Expected Benefit The result of this study will identify level of knowledge and practice towards Breast Self Examination among nursing student and how frequent they practice the right steps. This study also will deliver the knowledge about right BSE to the student for them to apply on themselves and to expose the knowledge to the community. 1.5.4 Significance of project Breast cancer is easier to threat the earlier it is found. There is evidence that women who correctly practice Breast Self Examination (BSE) monthly can detect a lump in the early stage of its development, and early stage of its development, and early diagnosis has been reported to influence early treatment and to yield a better survival rate. Unfortunately , despite the benefit of regular BSE ,a few women actually examine themselves , in fact , majority does not even know how to do BSE and opinion conflict about the value of BSEmen who correctly practice Breast Self Examination (BSE) montly can detect a The aim of this study was to investigate the level of knowledge and practice towards Breast Self Examination among nursing student and how frequent they practice the right steps. This study also will deliver the knowledge about right BSE to the student for them to apply on themselves and to expose the knowledge to the community. 1.5.5 Scope of the project Reference population : Student Nursing attending Medical and Health Science Faculty in UnisZA, Kuala Terengganu Study subject : Year 2 Nursing student in nursing Department at Medicine and Health Science in UnisZA during study period (1st January to 31 March ) 1.5.6 Definition of term Student anyone who is learning or someone who attends an educational institution (Wikipedia and free encyclopedia ) Nurse is a healthcare professional, who along with other health care professionals, is responsible for the treatment, safety, and recovery of acutely or chronically ill or injured people, health maintenance of the healthy, and treatment of life-threatening emergencies in a wide range of health care settings (Wikipedia and free encyclopedia ) Knowledge expertise, and skills acquired by a person through experience or education; the theoretical or practical understanding of a subject Practice a method of learning by repetition Breast Self Examination is a method of finding abnormalities of the breast, for early detection of breast cancer. The method involves the woman herself looking at and feeling each breast for possible lumps, distortions or swelling. Chapter 2 Literature Review According to Israa M. Alkhasawneh et al (2008) It is therefore important for nurses as educators to have appropriate information and positive attitude toward early detection of breast cancer. The provision of cancer screening behaviour can be complicated by the fear and uncertainty associated with cancer. Nurses knowledge and awareness of breast cancer screening behaviour would impact patients behaviour by increasing their awareness. Forgetfulness is the most important reason for not performing BSE. Furthermore, the fear of finding a mass, not having self-confidence, not being knowledgeable about how to perform BSE, laziness, and the absence of breast cancer in the family were the other reasons students refrain from the procedure. The main reasons for not performing BSE in the current study also were not know how to perform the examination, not having any previous problems in the breast, forgetfulness, and laziness. Sakine Memis (2009) Nursing profession is one of them, and it is very important for self carefulness to be able to recognize the signs of their own illness. Breast self-examination is an examination that should be perfect for nurses. They have the knowledge of the clinical signs of Breast Cancer and of the examination technique, and they can do it themselves without consulting a physician. Furthermore, they are especially aware of the importance of the early detection of breast cancer for a successful treatment. It has been shown that confidence in ones BSE ability is strongly correlated to BSE practice in the general population. For more emphasis of BSE occurs in the work place and in undergraduate and postgraduate courses, nurses, teaching of BSE to clients may be increased. Also, the provision of BSE educational programs is necessary to increase nurses knowledge, confidence, performance, and teaching of BSE.( Ali Abu-Salem 2007) It is supported by Agghababai sodabeh et al (2006) the nurses have a role for health and nature of the nurse client relationship facilitates opportunities for health education. Breast awareness will not necessarily translate into women becoming more familiar with their own breast tissue, unless they are encouraged to look at and touch the breast as a normal part of self -care health behavior. Regarding to G Ertem, A Kocer a positive correlation was found between nursing work experience and their practice alongside BSE medical professionals. Almost all the nurses knew how to conduct BSE, but did not prioritize practicing it. Nurses have knowledge about breast cancer screening behavior, such as BSE, the rates of performance are not adequate. It is therefore recommended that to increase rates of regular breast cancer screening behavior, mass health protective programs be conducted, especially for female health workers who undertake the responsibility of raising breast cancer prevention and awareness in society.. (2009) Dr.Osama et al Positive correlations were found between nursing work experience and their practice in BSE as working nurses. Studies like these can enhance the knowledge regarding BSE among nurses and other medical professionals. (2007) According to Roupa Z1., et al (2002) the majority of the subjects in student nurse acknowledged the usefulness and the convenience of BSE in the early detection of the breast cancer. The majority of the subjects fail to perform BSE regularly. Considerable percentages of the subjects in student groups have insufficient knowledge of BSE. From Mehregen Hj Mahmoodi et al (2002) Seventy-five percent of the women knew about breast cancer prevalence, but only 27% knew that breast pain is not a symptom of breast cancer. Although 73% of women did know that contact with a relative with breast cancer could not lead to development of breast cancer, the respondents knowledge of risk factors of breast cancer was not satisfactory. With regard to womens attitudes toward BSE, the majority believed that it is not difficult and time consuming or troublesome (63% and 72%, respectively). Sixty-three percent of the respondents claimed that they know how to examine their breasts, but only 6% performed BSE monthly. From study Ozgul Karayurt (2008), 6.7% of the students were performing BSE monthly and 20.3% of the students were performing BSE irregularly. Students knowledge about BSE might have affected their monthly BSE performance. However, only a small number of students who had knowledge about the BSE procedure were performing BSE monthly. The most common reasons for not doing BSE were not knowing how to perform BSE (98.5%), not expecting to get breast cancer (45.6%) and not having a close relative with breast cancer (42.9%).Consistent with the results of this study, in many studies, students noted that they did not perform BSE because they did not know how to perform it [4,29], and that they did not have a family history of breast cancer Chapter 3 Research methodology This chapter contains the research design adopted for the study, a description of the study site, study population, sampling and sample size, data collection tool and method, data analysis, inclusion and exclusion criteria; and ethical considerations. 3.2 Research design A cross- sectional quantitative study was conducted to determine the knowledge and practice of breast self examination. 3,3 Sample size 40 student of year 2 nursing student in department of nursing are selected as a sample for this study 3.4 Sample criteria inclusion criteria female age 18 years above year 2 student exclusion criteria male below 18 years year 1 and 3 student 3.5 Data collection tool and method Data collection was accomplished using administered questionnaire (See appendix A). The questionnaire was divided into 3 major parts. Part 1 Socio demographic data: age, education level, marital status, and number of children.. Coding for part 1: Question 1: Age Question 2: primary = 1, secondary = 2, tertiary = 3, none = 4 Question 3: Single = 1, married = 2, divorced = 3, widowed = 4, separated = 5 Question 4: none = 1, 1-5 = 2, 6-10 = 3, >10 = 4 Part II : Knowledge of breast self examination: Coding for part II Question 5 : S 1- S 5 True = 2 (correct answer ) False = 0 Question 6: S 1 S 10 True -2(correct answer) False = 0 Question 7: S1 true = 2 (correct answer) false = 0 S2 S4 False 2 (correct answer ) , True 0 Question 8 : S1- S3 True =2 (correct answer), false = 0 Question 9 : Yes = 0 No= 2 (correct answer) Question 10 : Yes = 2 (correct answer) No= 0 Question 11 : Yes = 2 (correct answer) No= 0 Question 12 : Yes = 2 (correct answer) No= 0 Question 13 : Yes = 2 (correct answer) No= 0 Question 14 : Yes = 2 (correct answer) No= 0 Question 15 : Yes = 2 (correct answer) No= 0 Question 16: S 1 S 3 True -2(correct answer) False = 0 Question17: S 1 S 2 True -2(correct answer) False = 0 Part 111 : Practice Question 18: Yes = (Practice), no (not practice) Question 27.1: monthly- 1 , every six month 2, yearly- 3 , never practice- 4 Question 27.2: forgetting = 1, not sure how to do it = 2, difficult to perform = 3, never taught how to do it = 4 Data collection was done by the researcher, from Feb. 2011 to Mac 2011 , which is a period of one months. 3.6 Data analysis Questionnaires were coded to suit computer statistical package and data was imported into Statistical Package of Social Sciences (SPSS). Analysis was done using SPSS to calculate mean, standard deviation and frequency distribution according to knowledge and practice of breast cancer examination. Questions to assess the knowledge of breast self examinations and the knowledge questions were and the scores were categorizes as follows: 34 = high. The total marks for the knowledge are 70 3.7 Ethical considerations The research was commenced only after receiving the approval of the Research Ethics Committee (MREC) of the Faculty of Health Sciences- University Technology Mara, and the local authority from Dean, Medicine and Health Science Faculty UniSZA, written informed consent was sought from the study participants at the commencement of the study. The aims and objectives of the research were introduced to the participants.. They were informed that their participation was voluntary and they were free to decline from the study at any stage. Participants were assured of their confidentiality while they participated in the study and privacy was maintained by not writing their names on the question CHAPTER 4 PRESENTATION OF RESULTS 4.1.1 Socio-demographic characteristics of the respondents Forty female student nursing student are participate in this study .the table of the social demographic characterized are shown on table 1. Majority of the female nursing student are same age, single and same educational level because they are join nursing course after Malaysian Certificated Education (SPM) Table 1 Variables Frequency % Age 0 0% 20 and above 40 100% Education level Secondary 0 0 Tertiary level 40 100% Marital status Single 40 100% Married 0 0 Widowed 0 0 Number of children none 0 0 1-3 0 0 4-5 0 0 >6 0 0 4.1.2 Level of knowledge 4.1.3 Symptoms of breast cancer Very few of the respondents that only 5 (12.5% ) knew that pain is not a symptom of breast cancer. Majority of the respondents 37 (92.5%) knew that lump in the breast is a symptom of breast cancer. The commonest presentation of breast cancer which is a painless breast lump only a third 16(40%) of the respondents knew about it. A very of the respondents 4 (10%) knew about nipple discharge as a symptom of breast cancer, while more of the respondents 35(87.5%) had knowledge on skin changes as shown on table 2 below. Symptom True % False % Pain in the breast 35 87.5 5 12.5 Lump in the breast 37 92.5 3 7.5 Painless lump 16 40 24 60 Nipple discharge 4 10 36 90 Skin changes 35 87.5 5 12.5 (Correct answer are highlighted in bold) 4.1.4 Risk factor of breast cancer Only one of the respondents 1(2.5%) did not knew about family history of breast cancer as a risk factor. That is same with risk factor where only one of the respondents 1(2.5%) knew about never given birth as a risk factor for breast cancer. This also applied to having many children, only one third of the respondents 11(27.5%) knew that it was not a risk factor. More than half of the respondents 30 (75%) knew that advancing age is also a risk factor for breast cancer. More than a half of the respondents 37(92.5%) knew that breast feeding is not a risk factor for breast cancer. Very few of the respondents 5(12.5%) had knowledge that oral contraceptive is a risk factor for breast cancer. Only one of the respondents 1(2.5%) knew that tobacco intake as well as excessive alcohol intake are risk factors for breast cancer respectively. One third of the respondents 12(30%) knew that high dietary intake is a risk factor. Being obese as one of the risk factor and more than half of the respondents 28(70%) knew about it. Thin women is not a risk factor for breast cancer only 19(47.5%) knew about it, as shown on the table 3. Statement true % false % Family history 39 97.5 1 2.5 Never giving birth 1 2.5 39 97.5 Having many children 29 72.5 11 27.5 Advancing age 10 25 30 75 Breast feeding 37 92.5 3 7.5 OCP 5 12.5 35 87.5 Tobacco intake 1 2.5 39 97.5 Excessive alcohol intake 1 2.5 39 97.5 High dietary fat intake 12 30 28 70 Obesity 28 70 12 30 Thin women 21 52.5 19 47.5 (Correct answer are highlighted in bold) 4.1.5 Protective factor of breast cancer Table 4 below shows the protective factors for breast cancer, All of the respondents 40(100%) and most of them knew that breast feeding is a protective factor for breast cancer and they also knew that not to be a breast feeding is not protective factor for breast cancer . The fact that you are young you are protected from the breast cancer, however half of the respondents 22(55%) knew about it. Having first child at older age almost half of the respondents 17(42.5%) knew that it is not a protective factor. Table 4 statement true % false % Breast feeding 40 100 0 O Not to breast feeding 6 15 34 85 Young age 22 55 18 45 Erderly primid 23 57.5 17 42.5 (Correct answer are highlighted in bold) 4.1.6 Method can detect breast cancer Majority of the respondents 39(97.5%), and 38(95%) knew that breast self-examination, clinical breast examination and mammogr

John Jays Hammond JR. :: essays research papers fc

John Hays Hammond, JR. John Hammond was one of the greatest electrical and mechanical inventors of his time. The things he invented during his lifetime impacted history a great deal. According to John Pettibone, John Hays Hammond, Jr. was born in 1888 in San Francisco, California (Pettibone 1). Most of his life Hammond was known as Jack. He was the second son and namesake of a world-famous mining engineer, who was the friend, confidant, and almost running mate of William Howard Taft. Jack’s father grossed a supposed one million dollars a year as well as bonuses at the South African gold and diamond fields where his father relocated his family in 1893. While in South Africa, Jack’s father got imprisoned by accident and in prison got really sick. Mark Twain was on tour of Africa and visited the prison and soon afterwards the Hammond family moved to recuperate in England (Dandola1-2). Young Hammond became devoted to studying life in the past and castles after his family relocated to England in 1898. At the beginning of the century his family moved back to the United States. Years later, as a marriage gift for his wife Irene, Hammond started building a medieval castle home in Gloucester, MA. In 1929 the couple took up residence in the castle and in 1930 revealed it as a museum (Pettibone 1). To invent, John Hays Hammond would at no time have to look far for ideas. He was born into an educated family in 1888 and some of the family’s associates included Nikola Tesla, the Wright brothers, and Thomas Edison. Hammond was both a realistic and fanciful inventor; his attractions varied from culinary and music to torpedoes and electronics (John 1). In New Jersey where John Hammond enlisted at the Lawrenceville School in 1903, his first invention came along. To elude the school’s 8:00 PM rule for lights out, Hammond was delighted to install into a lot of his friend’s dorms a sensor and an over current protection device that automatically turned off the rooms lighting as the door was opened. Hammond was disappointed years afterwards, that he had not listened to Edison’s advice, when a device similar to his became commonplace in vehicles and refrigerators. Edison had told him: Patent all your ideas, and get yourself a good lawyer (John 1). Jack never lost interest in medieval history, which became one of his passions after he was exposed to castles while enrolled in an English prep school.

Museu de Arte Contemporânea de Niterói Essay

The Niterà ³i Contemporary Art Museum (Museu de Arte Contemporà ¢nea de Niterà ³i — MAC) is situated in the city of Niterà ³i, Rio de Janeiro, Brazil, and is one of the city’s main landmarks. It was completed in 1996. Designed by Oscar Niemeyer with the assistance of structural engineer Bruno Contarini, who had worked with Niemeyer on earlier projects, the MAC-Niterà ³i is 16 meters high; its cupola has a diameter of 50 metres with three floors. The museum projects itself over Boa Viagem (â€Å"Bon Voyage,† â€Å"Good Journey†), the 817 square metres (8,790 sq ft) reflecting pool that surrounds the cylindrical base â€Å"like a flower,† in the words of Niemeyer. A wide access slope leads to a Hall of Expositions, which has a capacity for sixty people. Two doors lead to the viewing gallery, through which can be seen theGuanabara Bay, Rio de Janeiro, and Sugarloaf Mountain. The saucer-shaped modernist structure, which has been likened to a UFO, is set on a cliffside, at the bottom of which is a beach. In the film Oscar Niemeyer, an architect committed to his century,[1] Niemeyer is seen flying over Rio de Janeiro in a UFO which then lands on the site, suggesting this to be the origin of the museum. The MAC Scandal was a political scandal that occurred when the mayor Joà £o Sampaio inaugurated the Niterà ³i Contemporary Art Museum. The MAC is located on a hill slope that had locked construction rights set by the city council. Therefore in December 1996, the new mayor, Jorge Roberto Silveira sent a project to the city council to obtain the rights to construction in that area. The project was accepted in only two days, giving permission to build buildings up to 40 metres (130 ft). The city council did not know that days before, Zeca Mocarzel, sub-mayor of the Niterà ³i’s Oceanic Region (of Jorge Roberto Silveira’s government) bought the lands at a very low price, claiming to the old owner that the region was locked and nothing could be done there. So he bought the area and, after the inauguration of the MAC which substantially increased the property values of the nearby areas, later sold the land for more than 5 million reals, approximately 1,250,000 US dollars at the time (2,720,000 dollars today). Because the scandal occurred just before Christmas, the people of Niterà ³i said that it was Jorge Roberto Silveira, Zeca Mocarzel and Joà £o Sampaio’s (long-time Niterà ³i’s politicians) â€Å"Christmas present†.

Jane Eyre’s Struggle Between Conscience and Passion Essay

People can be held prisoner by their own feelings in an emotional box that confines them and controls them. Passion is the powerful, driving emotion that penetrates these feelings and compels one to break free of the box detaining them. In other words, passion is the motivation that drives one to take action against the shackles of their situation to create change in their life. All people have these passions, but what happens when these passions go against one’s conscience? A person’s conscience values things, like passions, as right or wrong, important or not important, or, significant or not significant. Thus, one’s conscience is like a barrier to one’s passions, and therefore, there is a constant struggle between the two. This internal struggle is prominent within Jane Eyre, the main character in Charlotte Bronte’s novel Jane Eyre. Jane’s conscience tells her to marry the one she loves, but her passion for freedom and equality conflicts her and creates for her an internal struggle. In the final chapters of the novel, Jane’s conscience eventually defeats her passion for individualism, completing her internal journey and creating a victorious conclusion. Jane grew up in the Victorian Era in England, and era in which women faced much inequality and prejudice. This is the box that confines Jane throughout most of the novel, and ignites her passion to break free of it and be an equal, individual woman. Jane expresses this on page 129 and 130 of the novel as she states, â€Å"Women are supposed to be very calm generally; but women feel just as men feel; they need exercise for their faculties and a field for their efforts as much as their brothers do; they suffer from too rigid a restraint, too absolute a stagnation, precisely as men would suffer; and it is narrow-minded in their more privileged fellow-creatures to say that they ought to confine themselves to making puddings and knitting stockings, to playing on the piano and embroidering bags. It is thoughtless to condemn them, or laugh at them, if they seek to do more or learn more than custom has pronounced necessary for their sex.† Thus, Jane does not want to be the property of a man because it interferes with her passion to be free. When Jane meets Mr. Rochester though, she develops a liking towards him, which eventually turns into a love for him. As a result, a struggle is born between Jane’s conscience, which is telling her to marry  Mr. Rochester, and her passion to be an equal individual woman. In the end, Jane realizes that loving Mr. Rochester is more important than continuing her rebellion against the constraints of society, and therefore, her conscience wins as she happily takes Mr. Rochester’s hand in marriage. The triumph of Jane’s conscience adds a victorious conclusion to the end of the novel, and completes Jane’s internal journey as a whole. Jane began her life watching her aunt and cousins let injustices wash over them. By experiencing this, Jane developed a passion to rebel against society and a passion for individualism, which she states in the quote on page 68, â€Å"If people were always kind and obedient to those who are cruel and unjust, the wicked people would have it all their own way: they would never feel afraid, and so they would never alter, but would grow worse and worse. When we are struck at without a reason, we should strike back again very hard; I am sure we should – so hard as to teach the person who struck us never to do it again.† This passion pushes Jane to survive the difficulties she encounters growing up, but forces her reject some of the happiness in life at the same time. This rejection is heartbreaking for the reader as the novel progresses, and she rejects more and more happiness. So therefore at the end of the novel when Jane finally sacrifices her passion for happiness with Mr. Rochester, it creates a triumphant ending and completes Jane’s transformation from rebellion and rejection to openness and love. Throughout the novel, Jane is in a constant struggle between her passion for individualism and her conscience, which tells her to pursue happiness instead. This struggle is the centerpiece for the novel, and therefore when Jane finally decides to let happiness in, it creates an exalting, happy ending for the reader. This ending furthermore completes Jane’s internal journey from rebellion to acceptance and love, which also is a satisfying ending for a reader.

Dissertation on Histone Protein Segregation The WritePass Journal

Dissertation on Histone Protein Segregation Dissertation on Histone Protein Segregation IntroductionAim Material and MethodsReagents usedExperimental ProcedureResultsDiscussionReferences Related Introduction Histone Proteins are favourably the alkaline proteins which are present in the nucleus of the eukaryotic cell. They are the basic parts responsible for wrapping and organizing DNA into chromosomes in the nucleus. They are the principal protein elements of the chromatin and acts as a spool around which the DNA winds. In the nucleus of the cell the DNA is arranged in a very dense and super coiled style to form chromosomes. This is referred to as DNA packaging. (Isenberg, 1979) The unwound DNA in the chromosomes is very long and cannot be fit in the nucleus of the cell, hence these histone proteins brings about the DNA wrapping thereby reducing the dimensions of DNA in the cell. (Alberts B et al., 2001) Histones are primarily categorized into five major types. They are H1/H5, H2A, H2B, H3 and H4. They are categorized into two classes. They include core histones with H2A, H2B and H3 and linker histones with H1 and H5. Two of each of core histones unites to form one octameric nucleosome which is the fundamental sub unit of chromatin. (Bartova et al. 2008; Bonisch et al. 2008). Hence histones proteins form the nucleosomes directly. After extraction of chromatin from the cells it is like beads on string. The string is the DNA and the beads are the nucleosomes which are roughly disc shaped. The linker histone H1 separates the nucleosome with the other nucleosomes and also helps in bringing the adjoining nucleosomes for further super coiling. The octameric nucleosome is formed by two H2A, H2B dimers and a tetramer of H3, H4 histones. These core histones are comparatively analogous in structure. However their 3D structures are relatively different. The core histones have different protein sequence. The sequence for H2A Histone is SGRGK QGGKA RAKAK SRSSR AGLQF PVGRV HRLLR KGNYS ERVGA GAPVY LAAVL EYLTA EILEL AGNAA RDNKK TRIIP RHLQL AIRND EELNK LLGRV TIAQG GVLPN IQAVL LPKKT ESHHK AKGK. (Andreas and David, 1998) The sequence for H2B Histone is PEPAK SAPAP KKGSK KAVTK AQKKD GKKRK RSRKE SYSIY VYKVL KQVHP DTGIS SKAMG IMNSF VNDIF ERIAG EASRL AHYNK RSTIT SREIQ TAVRL LLPGE LAKHA VSEGT KAVTK YTSSK. (Andreas and David, 1998) The sequence for H3 Histone is ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP ATGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL VREIA QDFKT DLRFQ SSAVM ALQEA CEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA. (Andreas and David, 1998) The sequence for H4 Histone is SGRGK GGKGL GKGGA KRHRK VLRDN IQGIT KPAIR RLARR GGVKR ISGLI YEETR GVLKV FLENV IRDAV TYTEH AKRKT VTAMD VVYAL KRQGR TLYGF GG. (Andreas and David, 1998) The molecular weight of the core histone proteins are measured in the Daltons. The theoretical molecular weight for H2A histone protein is 13,990.28 Daltons, for H2B histone protein is 13,788.97 Daltons, for H3 histone protein is 15,272.89 Daltons and for H4 histone protein is 11,236.15 Daltons. (Kornberg, 1977;McGhee and Felsenfeld, 1980) The histone proteins play a significant role in chromosome stabilisation, gene regulation and expression. The main functions of histone proteins are condensing the DNA strands and chromatin regulation. These histone proteins form the nucleosome and they impart a structure to which DNA is twisted and causes to fit the bulky genomes of eukaryotes inside the nucleus of the cell. (Bartova et al. 2008; Bonisch et al. 2008). The histones also undergo post translational modifications which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have very long tails projecting from the nucleosome which can be covalently altered at numerous places and the alteration includes the acetylation, ubiquitination, methylation, phosphorylation, ADP-ribosylation and citrullination.(Bartova et al. 2008; Bonisch et al. 2008). The modification of histone includes the gene regulation, DNA repair and chromosome condensation. In this manner, histones can control gene expression, cell g rowth and proliferation. (Jenuwein and Allis 2001). The separation of the histone proteins is carried out by using the 1D SDS PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) method. In the gel electrophoresis method the charged molecules are segregated based on their physical properties like charge, mass, etc. by allowing them to pass forcedly through a gel matrix by an electric current. (Coligan et al., 2002).The proteins from the various complex protein mixtures are normally separated by this method by using the polyacrylamide gel. It is known as Polyacrylamide Gel Electrophoresis (PAGE method). In this method the main constituent is the acrylamide which is used in the preparation of the electrophoresis gels for the isolation of proteins. In order to form the cross linked polymer network, the acrylamide is combined with bisacrylamide with the polymerising agent like Ammonium Per Sulphate (APS). The polymerisation reaction is catalysed by TEMED (N,N,N,N-tetramethylenediamine) by the producing the free radicals by APS. The size of the pores and firmness of the gel matrix mainly depends on the ratio of bisacrylamide and acrylamide used and their total concentrati on. Sequentially these in turn depends up on the range of molecular weight of the proteins which has to be determined. The pore size of the gel matrix is contrary to the quantity of acrylamide used. Generally 12% of polyacrylamide gel has lesser pore size than 7% of polyacrylamide gel. Larger proteins are resolved with the gels with less amount of acrylamide and smaller proteins with more amount of acrylamide. And for the broad range of protein sizes, the special gels like Gradient gels are prepared by having less percent of acrylamide at the top/start and more percent of acrylamide at the bottom/end. The electrophoresis gels are mixed with the buffers which impart the conduction of electric current though the matrix. The solution is transferred to the gel cassette which is a thin space formed by placing two glass or plastic plates facing each other. After the gel is polymerised, the cassette is placed vertically into the electrophoresis tank containing the electrodes. The proteins are added in the wells from the top and electrophoresis is carried out during which the proteins are separated by the gel due to its sieving properties. In order to obtain the best possible resolution of the proteins a gradient gels are used. (Hames et al., 1990) There are different forms of PAGE available which are used for separation of different proteins based on different principles. They commonly include the native PAGE, SDS PAGE, 2D PAGE, etc. (Hames et al., 1990; Coligan et al., 2002). In case of the 2D PAGE (Two Dimensional Polyacrylamide Gel Electrophoresis) the proteins are isolated by isoelectric point in the first dimension and later by mass in the second dimension. This method offers the maximum resolving power for the protein analysis and it is a very significant method in proteomic research. (Hames et al., 1990) In the native PAGE, the proteins are isolated based on their size, net charge and shape. The electrophoretic migration is due to the fact that the most of the proteins possess a net negative charge in alkaline running buffers. As the negative charge density increases, the migration speeds of the protein increase. Simultaneously the frictional force of the gel matrix produces a sieving effect thereby reducing the protein movement based on their size and shape. Hence the smaller proteins face less frictional force and larger proteins face more frictional force. In this manner the various proteins are separated from a mixture. (Hames et al., 1990) In case of the SDS PAGE the gel is mixed with the buffer having the SDS (Sodium Dodecyl Sulphate). The SDS is heated with the proteins samples before electrophoresis so that the charge density of all proteins are almost equal. The SDS is an anionic detergent which denatures the proteins present in the sample and attaches strongly to the uncoiled molecule. In order to make sure that no quaternary or tertiary proteins structure remains generally a reducing agent like dithiothreitol (DTT) is also added as it ruptures the protein disulphide bonds. Hence when these samples are used in the electrophoresis, the proteins get separated based on their mass alone. In this method a set of proteins of known molecular weight is run aside the sample in the same gel cassette. They act as a reference from which the mass of the sample proteins is determined. They are called as Molecular Weight Markers. The electrophoresis is carried out by using the two gels for favourable results. They are the stacki ng gel and the resolving gel. The stacking gel is added over the top of the resolving gel. Apart from the low concentration of acrylamide in stacking gel, it has low pH and different ionic content than the resolving gel. This causes the proteins to get concentrated into a tight band during the early electrophoresis period before entering into the resolving gel. (Hames et al., 1990) Breast cancer refers to the hysterical growth of breast cells. The breast carcinoma is a malignant tumour. It occurs due to any abnormal genetic changes in the breast cell. Presently there is an increasing alarm regarding the high risk posed by various compounds with oestrogen like activity present in the environment. These various compounds includes the Phytoestrogens (e.g. genistein), food products like legumes, lentils, chickpeas, soybean, cereals, fruits, and vegetables, industrial contaminants, (e.g. bisphenol A) and polychlorinated biphenyls, organochlorine pesticides (e.g. endosulfan), etc. These substances have the ability to produce cancer mediated through estrogenic receptors. Some of these substances when treated with the MCF-7 cell line, MCF-10F (ERÃŽ ±Ã¢Ë†â€™/ERÃŽ ²+), MDA-MB-231 (ERÃŽ ±Ã¢Ë†â€™/ERÃŽ ²+) or MCF-10A (ERÃŽ ±Ã¢Ë†â€™/ERÃŽ ²Ã¢Ë†â€™) cells, increased the growth rate of these cell lines. This 3-4 folds proliferation was due to the up regulation of all the core h istone proteins which represents the increase in the chromatin content of the cells. The degree of proliferation of the cells indicates the level of the core histone proteins and was concentration dependent. Hence this indicates that histone proteins are used as indirect markers of breast cell proliferation. Therefore in the human breast cancer the histones are up regulated and cause cell proliferation mediated by the oestrogen receptors agents. Based on this it is possible to state that the core histone proteins serve as bio markers of (ER+) human breast cancer.(Zhu et al., 2009) Aim The main aim of this experiment is to resolve the given four human recombinant protein samples (H2A, H2B, H3 and H4) by using the one dimensional SDS Poly Acrylamide Gel Electrophoresis (1D SDS – PAGE). Material and Methods Equipments used The experiment uses the Bio Rad Mini PROTEAN 3 Cell gel electrophoresis unit. It consists of the electrophoresis tank, electrode chamber with a cathode and anode, two glass plates, gel cassette assembly, comb and the connecting cables. Power source, heater, micro centrifuge, pH meter, and the other general equipments like test tubes, conical flasks, etc were used. Reagents used The various reagents used in this experiment includes the Acrylamide/bisacrylamide (30%w/v/0.8%w/v), 3.0M Tris/HCl (pH – 8.8 for resolving gel stock), 0.5M Tris/HCl (pH – 6.8 for stacking gel stock), TEMED, SDS (10%w/v), Ammonium Per Sulphate (APS – 25%w/v). The running buffer is also used as 0.025M Tris/0.192M glycine/0.1% (w/v) SDS, pH 8.3. The other reagents used are sample buffer, Pre stained molecular markers, colloidal Coomassie Blue Stain Solution, Bromophenol Blue (0.5%w/v) and the given four human recombinant proteins (1 µg/ µl) [H2A, H2B, H3.3 and H4]. Experimental Procedure The SDS PAGE method is commonly used for the examination of the proteins because of its simplicity, speed and resolving capacity. The SDS PAGE is the Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis method. The various steps performed are as follows: Preparationof Gel Cassette Sandwich Firstly select a clean Spacer Plate and Short Plate and place them over one another and set them in the casting frame and lock the pressure cams from both the sides. It should be kept in such a manner that the short plate should face the front of the frame. After locking the gel cassette assembly check that both the plates are flush at the bottom. Then place the rubber gasket at the bottom of the casting stand and place the gel cassette sandwich assembly in the casting stand in such a manner that it is perpendicular to the level surface. After complete formation of the gel cassette assembly, add water into it to make sure that no leaking takes place. Then remove the water and wipe it with the filter paper. Set the comb into the assembled gel cassette and mark the level at 1 cm below the comb teeth and remove it, the resolving gel is poured up to this level. After preparation of the resolving gel pour it up to the mark into the assembled gel cassette in a very smooth way to prevent an y air bubble formation. Allow the gel to set for 45 minutes. After the gel has set wash its surface with distilled water and wipe with the filer paper and allow drying the area in between the glass plates for a minute. Then after this prepare the stacking gel solution and pour it above the resolving gel till the top of the plates. Then immediately place the comb in the stacking gel in gel cassette to form the wells. Allow the stacking gel to set for overnight. Preparation of Gels The gels used in SDS PAGE method are the resolving gel and the stacking gel. Both the gels consists of the acrylamide/bisacrylamide, distilled water, 10% SDS, TEMED and ammonium per sulphate (APS). However the resolving gel uses 3.0M Tris/HCl (pH 8.8) and the stacking gel uses the 0.5M Tris/HCl (pH 6.8). These gels are prepared by directly mixing all the reagents. However the TEMED and APS are added only when the gel is ready to be polymerised. In this manner the gels are prepared. Electrophoresis Module Assembly After the stacking gel has set, smoothly remove the comb. Then open the cams and remove the sandwich gel cassette and place it in the electrode assembly in such a manner that the short plate faces inward of the electrode assembly. Secure the electrode assembly by closing the two levers of the frame to form the inner chamber. Set this secured inner chamber into the Mini Electrophoresis Tank. Fill the inner chamber with the running buffer, however it is not overfilled. Then add around 200 ml of the running buffer is added into the Mini Tank. Sample Preparation and Loading Four recombinant histone protein samples are used in this experiment. They are H2A, H2B, H3.3 and H4. 1 µl of each of this protein samples (1 µg/ µl) is added to 20 µl of the Sample Buffer in the Eppendorf tubes and labelled properly. They are heated in a heater set at 100 º C for two minutes and then allowed to cool to room temperature. After the sample preparation, 20 µl of each of the histone protein is loaded into the wells carefully by using the gel loading tips. The sample loading should be very smooth and the tip should not puncture the well. Load the 2 µl of the pre stained molecular marker (Page Ruler) into the other well simultaneously to ensure the movement of proteins. Gel Electrophoresis After loading the histone proteins, mini tank is covered with the lid and the electric supply is given by the cable wires. Turn on the power supply and the start the gel electrophoresis at 200 Volts for about 35 minutes until the bromophenol blue dye has drifted to 1 cm from the bottom of the gel. During this time each histone protein based on their molecular weight is moved to their ends. Staining the Gels Once the electrophoresis is completed, switch off the power supply and take out the lid. Cautiously lift the inner chamber assembly and dispense the running buffer to avoid the falling of buffer before opening the cams. Then open the cams and take out the glass cassette sandwich. Open the sandwich with the help of gel releaser and take out the gel slowly. Immerse the gel in 30 ml of Colloidal Coomassie blue stain solution for half an hour with moderate shaking. During this time the staining occurs and once it is completed the gel is de stained with water and then incubated with water at room temperature for overnight for complete de staining. After this the gel is rinsed with water for the proteins bands to be seen clearly. Results Figure 1: SDS Poly Acrylamide Gel Electrophoresis of Histone Proteins. Bands show the migration of individual histone proteins. A pre stained molecular marker is included at the side for comparison. In the present experiment four human recombinant histone proteins were given for isolation and determination. From the standard pre stained molecular marker, each of the histone protein has to be determined by correlating with its band. The Figure 1 illustrates the SDS Poly Acrylamide Gel Electrophoresis of given four recombinant histone proteins (H2A, H2B, H3.3 and H4). In the figure the last column of bands refers to the standard pre stained molecular marker (Page Ruler) and the other four bands represents the respective histone proteins. The values beside the molecular marker indicate the molecular weight in kDa. Hence with reference to this standard values, the four given histone protein samples has to be determined. In case of the band of H2A histone protein, it is nearly 13,990 Da as it is below 15 kDa. Similarly in case of the band of H2B histone protein, it is nearly 13,789 Da. And in case of H3.3 histone protein, the band is clearly at the top of all the bands indicating it has the highest value and it is slightly above 15, so it may be around 15,273 Da. In the fourth histone protein H4, the band is at the far end when compared to the other three bands. However it is slightly above 10. Hence it may be around 11,236 Da. Therefore the molecular weights of the histone proteins were identified. However the standard molecular weight for the H2A is 13,990.28 Da, for H2B is 13,788.97 Da, for H3.3 is 15,272.89 Da and for H4 is 11,236.15 Da. (Kornberg, 1977;McGhee and Felsenfeld, 1980) Discussion The separation of proteins is mainly performed by the gel electrophoresis method. There are different types of gel electrophoresis methods used to separate different types of proteins. However in the present experiment the isolation of histone proteins is mainly carried out by 1D SDS PAGE method. This method is a one dimensional method and it separates the proteins principally by their molecular weight. It makes use of the ionic detergent Sodium Dodecyl Sulphate (SDS). The poly acrylamide gel is mixed with SDS because it denatures the proteins and binds to them to make them approximately evenly negatively charged. This is done with the aid of heating and generally a reducing agent like dithiotheritol (DTT) in order to break the protein disulphide bonds. This removes the risks of presence of any tertiary or quaternary proteins. As a result when electric current is passed through a power supply, all the SDS bound proteins of the sample will drift in the gel matrix to the anode, thereby separating the proteins based on their molecular weight alone. The proteins with low molecular weight moves fast through the gel compared to the proteins with high molecular weight due to the sieving effect of the gel matrix. Therefore in this experiment a set of four human recombinant histone proteins (H2A, H2B H3.3 and H4) were given to separate them based on their molecular weight. Hence these protein samples were taken and were subjected to the SDS PAGE method. In this method firstly the gel cassette assembly was formed by using the two glass plates (Spacer Plate and Short Plate) in a proper manner such that the short plate is facing front of the frame. After assembling the gel cassette it was filled by the gels which were prepared just before use. Firstly the resolving gel was poured and after it got set for about 45 minutes, the stacking gel was poured and comb was placed in it and it was allowed to set for overnight. The pouring of gels was carried out very carefully and slowly in order to prevent any air bubble formation. The appropriately set gel with the well defined wells is selected and gel cassette assembly was set in the electrode unit in the Mini Electrophoresis Tank. The running buffer was added properly and the sample histone proteins were loaded very cautiously. Before loading the protein samples, they were heated for about two minutes. Along with the samples, the pre stained molecular marker was also loaded. And the power supply was given and the electrophoresis was carried out for 35 minutes at 200V. During this time the bromophenol blue dye moves to 1cm from the bottom of the gel. After this the power supply was switch off and very carefully the gel was removed from the gel cassette sandwich assembly and was dipped in the staining solution like Colloidal Coomassie blue stain solution for about half an hour with occasional shaking. After staining, the gel is removed and de stained with distilled water by incubating it for overnight. Then it was rinsed properly to visualise the bands for each of the histone proteins. The pre stained molecular marker of known molecular weight was also run beside the given histone samples in the same gel. It acts as a standard or reference. For all the given four human recombinant histone proteins the bands lie in between the 10 and 15 kDa as per the marker. In situation of H2A histone protein, the band obtained is just below the 15 kDa band in the standard. Hence its molecular weight is around 14 kDa. In case of the H2B histone protein, the band is obtained also below 15 kDa but a little lower than H2A, hence it should be less than 14 and should be around 13.7 kDa. In case of H3.3 histone protein, the band is just above the 15 kDa and it should be around 15.3 kDa and for the H4 histone protein the band is quite low and just above the 10 kDa band; hence it should be around 11.3 kDa. The data obtained in this experiment is fairly correct as compared to the standard theoretical data. The theoretical molecular weight for H2A histone protein is 13,990.28 Daltons, for H 2B histone protein is 13,788.97 Daltons, for H3 histone protein is 15,272.89 Daltons and for H4 histone protein is 11,236.15 Daltons. (Kornberg, 1977;McGhee and Felsenfeld, 1980). Hence the results obtained in this experiment are reasonably acceptable and can be used for further studies. If the mixture of these histone proteins were need to be separated by the same method (SDS PAGE), then the expected result would not be accurate and proper. And the band for the individual histone protein may not be clear. It is because all the given four human recombinant histone proteins have approximately equal and very close molecular weights. And the values for all the four histone proteins lie between 10 to 16 kDa. Hence the bands would be very close to each other. If any flaws or any possible inaccuracy occurs during the experiment, then there is a possibility that the bands may be wrong. Hence the results would be wrong. Therefore there is very less possibility that the results would be accurate and also for the correct identification of the bands. However, if the experiment is carried out perfectly, then in such case the order of bands would be H4, H2B, H2A and H3.3 in the increasing order of their molecular weights. It is resolved with the help of the standard molecular mar ker which acts as a reference. Therefore, if mixture of these four histone proteins were to be separated by this method very accurately, then the results may be correct. However, for the separation of this mixture of four histone proteins another laboratory method is available. In this method, the groups (H2A, H2B) and (H3, H4) are separated first by using NaCl and hydroxylapatite chromatography. (Richard and Gary, 1979) The H2A and H2B group are the slight lysine rich group and H3 and H4 is the arginine rich group. This method was introduced by Van der Westhuyzen and Von Holt. In this method, firstly the individual groups were separated. However, the given mixture was treated with 0.63 M NaCl and 0.1 M potassium phosphate before the mixture sample is loaded on to the hydroxylapatite. This is to remove any contamination with the other linker histone proteins like H1 and H5. Then it was subjected to chromatography to obtain the core histone groups (H2A, H2B); (H3, H4). After this the samples obtained are subjected to SDS PAGE method for further separation. Finally the core histones were separated. (Richard and Gary, 1979) References Ahmad, K. and Henikoff, S. (2002). Histone H3 variants specify modes of chromatin assembly Proc. Natl. Acad. Sci. U. S. A. 99 Suppl 4, 16477-16484. Andreas, D.B. and David, L. (1998 Oxford University Press). Nucleic acids research. Histone Sequence Database: new histone fold family Vol. 26, No. 1, 372-375. Bartova, E. Krejci, J. Harnicarova, A. Galiova, G. and Kozubek, S. (2008). Histone modifications and nuclear architecture: A review J. Histochem. Cytochem. 56, 711-721. Bonisch, C. Nieratschker, S.M. Orfanos, N.K. and Hake, S.B. (2008). Chromatin proteomics and epigenetic regulatory circuits. Expert Rev Proteomics.5, 105–119. Coligan, J.E., et al. , Eds. (2002). Electrophoresis, In Current Protocols in Protein Science, John Wiley and Sons, Inc. New York. 10.0.1-10.4.36. Hames, B.D. and Rickwood, D. Eds. (1990) Gel Electrophoresis of Proteins: a Practical Approach, 2nd ed. Oxford University Press, New York. Isenberg, I. (1979). Histones. Annu. Rev. Biochem. 48, 159-191. Jenuwein, T, and Allis, C.D. (2001). Translating the histone code. 293. 1074–1080. Kerenyi, L. and Gallyas, F. (1973). Errors in quantitative estimations on agar electrophoresis using silver stain Clin. Chim. Acta 47, 425-436. Kornberg, R. D. (1977). Structure of chromatin Annu. Rev. Biochem. 46, 931-954. Marino-Ramirez, L. Jordan, I. K. and Landsman, D. (2006). Multiple independent evolutionary solutions to core histone gene regulation Genome Biol. 7, R122. McGhee, J. D. and Felsenfeld, G. (1980). Nucleosome structure Annu. Rev. Biochem. 49, 1115-1156. Richard, H.S. and Gary, F. (February 1979). Nucleic acids research, oxford journals. A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite Volume 6 Number 2, 689-696. Rà ¼chel, R. Steere, R. L. and Erbe, E. F. (1978). Transmission-electron microscopic observations of freeze-etched polyacrylamide gels? Journal of Chromatography A 166, 563-575. Shapiro, A. L. Vinuela, E. and Maizel, J. V.,Jr. (1967). Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels Biochem. Biophys. Res. Commun. 28, 815-820. vanWert, J. M. Wolfe, S. A. and Grimes, S. R. (2008; 2008). Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes J. Cell. Biochem. 104, 1087-1101. Weber, K. and Osborn, M. (1969). The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis J. Biol. Chem. 244, 4406-4412. Zhu, Z. Edwards, R. J. and Boobis, A. R. (2009). Increased expression of histone proteins during estrogen-mediated cell proliferation Environ. Health Perspect. 117, 928-934. Version 112,113, 114,115 and 116. UniProtKB/Swiss-prot release 2010_04 P16104

Listado completo de profesiones para sacar la visa TN

Listado completo de profesiones para sacar la visa TN Los mexicanos que tienen una oferta de trabajo en los Estados Unidos pueden obtener la visa TN. Para poder beneficiarse es necesario ser profesional y tener una titulacià ³n universitaria o, en ciertos casos, una certificacià ³n o licencia o un mà ­nimo de aà ±os de experiencia. Pero no todas las profesiones permiten sacar la visa TN, que se creà ³ como consecuencia del TLC entre Mà ©xico, Estados Unidos y Canad.   Sà ³lo las profesiones que estn incluidas en una lista, que se detalla a continuacià ³n, con especificacià ³n de estudios que se piden o alternativas, cuando es posible.   Profesiones para la visa TN para las  que es necesario ser egresado universitario Asistentes de investigacià ³n (sà ³lo para trabajar en colleges y universidades)Arquitecto de paisajesBibliotecarioConsejero vocacionalConservacionistaEconomistaEstadà ­sticosMatemticosNutricionistaPlanificador urbanoProfesor (sà ³lo en universidades o colleges o seminarios), para maestros: visa J-1 o H-1.SilviculturistasTerapista recreacionalTrabajador social Cientà ­ficos egresados que trabajan como: AgriculturistasAgronomistasApiculturistasAstrà ³nomosBià ³logosBioquà ­micosCientà ­ficos especialistas en animalesCientà ­ficos del sueloEntomà ³logosEpidemià ³logosEspecialista avà ­colaEspecialistas en productos lcteosEspecialistas en reproduccià ³n animalEstadà ­sticosFà ­sicosFitomejorador (plant breeder)GeneticistasGeà ³logosGeofà ­sicosGeoquà ­micosHorticultorMatemticosMeteorà ³logoQuà ­micosPatà ³logo de plantasTerapista recreacionalZoà ³logos Profesiones para las que se requiere ser egresado universitario o tambià ©n se admite otro tipo de credencial Abogado (titulacià ³n universitaria o miembro de colegio de abogados -bar- de Mà ©xico, Estados Unidos o Canad).Actuario (tà ­tulo universitario o credencial)Ajustador de reclamo de seguros por desastres (entrenamiento ms un tà ­tulo o tres aà ±os de experiencia)Analista de sistemas de computacià ³n (titulacià ³n universitaria, diploma post escuela secundaria o certificado post escuela secundaria ms tres aà ±os de experiencia)Arquitecto (tà ­tulo universitario o licencia)Consultor en Administracià ³n (titulacià ³n universitaria o cinco aà ±os de experiencia)Dentista (licencia o grado profesional)Dietistas (titulacià ³n universitaria o licencia)Diseà ±ador de interiores (titulacià ³n universitaria, certificado o diploma ms tres aà ±os de experiencia)Diseà ±ador grfico (titulacià ³n universitaria o diploma post escuela secundaria ms tres aà ±os de experiencia)Diseà ±ador industrial (titulacià ³n universitaria, certificado o diploma ms tres aà ±os de experiencia)En fermeras/os (registered nurses). Titulacià ³n o licencia.Escritor de publicaciones tà ©cnicas (titulacià ³n o diploma o certificado ms tres aà ±os de experiencia) Farmacà ©uticos (titulacià ³n o licencia)Gerente de hotel (tà ­tulo universitario o diploma o certificado ms tres aà ±os de experiencia)Guardabosque (titulacià ³n universitaria o licencia)Ingeniero (titulacià ³n universitaria o licencia)Mà ©dicos (M.D. o licencia, pero sà ³lo para enseà ±ar o trabajar en investigacià ³n). Para trabajar como mà ©dicos son otros los requisitos.Psicà ³logos (titulacià ³n o licencia)Tecnà ³logo de laboratorio mà ©dico (titulacià ³n universitaria, o certificado o diploma ms tres aà ±os de trabajo)Terapista fà ­sico (titulacià ³n o licencia)Terapista ocupacional (titulacià ³n universitaria o licencia)Topà ³grafo (titulacià ³n universitaria o licencia)Veterinario (grado profesional o licencia) Casos en los que no se pide titulacià ³n ni certificacià ³n o licencia Tà ©cnicos cientà ­ficos Siempre y cuando està ©n trabajando con profesionales en los campos de Astronomà ­a, Biologà ­a, Ciencias Agrà ­colas, Ingenierà ­a, Geologà ­a, Geofà ­sica, Fà ­sica, Meteorologà ­a, Quà ­mica o Silvicultura. Dà ³nde encontrar ms informacià ³n sobre los requisitos para esas profesiones Todos los datos necesarios estn incluidos en el tratado de NAFTA (TLC) en el Anexo 1603, apà ©ndice 1603.D.1 (informacià ³n en inglà ©s) y tambià ©n en el 8 C.F.R.  Ã‚ § 214.6. A tener en cuenta La visa TN es una gran alternativa a la H-1B, que es la que utilizan muchos profesionales pero tiene el gran inconveniente de que sà ³lo se pueden aprobar un nà ºmero fijo por aà ±o fiscal y el nà ºmero de solicitudes es ms grande que el de visas que se pueden dar. A consecuencia de ello, es frecuente que tenga que celebrarse una loterà ­a para determinar quià ©n es el ganador de la H-1B.  Poder obtener una visa TN elimina ese problema. Para tramitar la visa TN se sigue un procedimiento especà ­fico que es diferente al de otras visas de trabajo temporal e incluso al que siguen los canadienses, que tambià ©n pueden disfrutar de estos visados al amparo del Tratado de Libre Comercio entre Mà ©xico, Estados Unidos y Canad, que fue cuando se crearon. Por à ºltimo, tener en consideracià ³n que es requisito fundamental para la TN tener una oferta de trabajo en Estados Unidos, no puede utilizarse para el auto-empleo. Opciones en Canad Los profesionistas  mexicanos que desean trabajar en otro paà ­s pueden considerar entre sus opciones no sà ³lo a los Estados Unidos sino que tambià ©n Canad puede resultar ser una buena alternativa. Destacar que el rà ©gimen migratorio en este à ºltimo paà ­s es diferente y es posible emigrar al amparo de las leyes de Canad que aplican a todo el paà ­s o tambià ©n aprovechando las oportunidades que brindan las distintas provincias.   Por ejemplo, este es un listado de ms de 300 profesiones que permiten emigrar a Canad siguiendo trmites exprà ©s. Por otro lado, las provincias brindan tambià ©n buenas oportunidades para emigrar, como por ejemplo Quebec. Si bien las plazas disponibles se agotan muy pronto para los candidatos que no hablan francà ©s nivel medio. En todo caso, son opciones que puede resultar muy interesante explorar. Este es un artà ­culo informativo. No es asesorà ­a legal

Apa Format Cheat Sheet

Space once after periods on the reference list | Title Page Format   p. 229Example: p. 41Exception: You do NOT need to include an Author Note| The title page should include the following five items:   1. Header flush left:Running head: ABBREVIATED TITLE (maximum 50 characters)   2. Page number flush righttitle page is page 1  Ã‚   3. Centered and in the upper half of the page (double spaced): Title of PaperStudent NameAmerican Sentinel University| Body of Paper| 1. Header flush left:ABBREVIATED TITLE   2. Page number flush right   3. Center title on first line of first page  | Headingsp. 62| 4. Use at least one level of heading (Level 1), though depending on paper length, two levels of headings (Levels 1 2) may enhance readability Level 1Centered with bold font, upper and lower case lettersLevel 2Flush left, bold font, upper and lower case letters(See the APA manual for three additional levels of headings used for longer papers). | Introduction/Conclusion  pp. 27; 63| The first paragraph of all papers should introduce the reader to what the paper will be about, and include a purpose statement. It is assumed that the first paragraph is the introduction; therefore, do not use â€Å"Introduction† as a heading. Close the paper with a conclusion or summary that summarizes the key points of the paper. You do not need to say â€Å"In conclusion† because the heading says that. For the â€Å"how-to’s† of writing a great intro and conclusion, see: http://www. princeton. edu/writing/center/resources/introconcl. pdf| Writing Style  p. 69; also see: http://blog. apastyle. org/apastyle/2009/09/use-of-first-person-in-apa-style. html| Writing in the third person is standard at American Sentinel, though there are exceptions (check with your instructor); avoid use of person all together when possible. Chapter 3 of the APA manual has some great writing tips! Here are a few additional resources students have found helpful in writing papers:    1. Principles of Composition 2. Correct use of Punctuation 3. How to do a Final Polish/Edit of your Assignment 4. Mission Critical—Critical Thinking Interactive Tutorial| Quotation 40 Words or More   pp. 92; 171-173|   Use block quotationno quotation marks, page number preceded by the period at the end of the last sentence. Note: Limit use of direct quotations! Better to paraphrase and cite. | Writing in a Series (Seriation) pp. 63-65| Use numbers to indicate an ordered position (e. . , of importance): 1. 2. Can use bullet points if there is no order of importance or chronology: * * As part of a sentence: Options for writing a list in APA format includes (a) numbers, (b) bullet points, and (c) lower case letters in parenthesis, depending on how the list is used. | Writing Numbers pp. 111-113| Numbers expressed in numerals: * 10 and above * Precede a measurement (e. g. , 5 mg)Numbers exp ressed in words: * Below 10 * Beginning a sentence or heading| Citing In Text (Preferred citation format listed)   Note the period follows the parenthesis. There must be a citation on the reference page for each in text citation. | Paraphrasing   p. 170| †¦(Young, 2010). | Direct Quotationp. 170-172| †¦(Young, 2010, p. 3). Note: Limit use of direct quotations! Better to paraphrase and cite. | Secondary source p. 178| †¦ (York, as cited in Johnson, 2009). Note: Only Johnson goes on the reference page. | No Page Numbers p. 171-172|   Give paragraph: (Brown, 2010, para. 3) ORGive section: (Davis, 2010, Abstract section). | No Date   Ã‚  p. 185| †¦(Smith, n. d. ). | Personal Communication   p. 178|   (J. Jones, personal communication, July 12, 2010). Note: Do not include on the reference page | Citing Multiple Authors   No need to memorize, just keep this list handy. pp. 175| Author Number| In Text Citation | Reference List Citation| 2| List both authors every time†¦(Author Author, year)  |   Ã‚  Ã‚  List all authors| 3-5| Cite all authors the first time: †¦(Author, Author, Author, year)Subsequently, cite only the first author followed by et al. †¦(Author et al. , year)  |   Ã‚  Ã‚  List all authors  | 6 or more| First time and every time, cite only the first author followed by et al. Author et al. , year)  Ã‚  Ã‚  |   Ã‚  List all authors| 8 or more| | Include the first seven authors’ names, then insert three ellipses, and add the last author’s name, like this:Author, I. , Author, I. , Author, I. , Author, I. , Author, I. , Author, I. , Author, I. ,†¦Author, I. (year). | Reference Citations Always compare to an example as you build and proofread your references. Every reference needs an in text citation. pp. 180-183; examples: pp. 198-224| General| 1. Reference list begins on a new page, with the word Reference centered at the top of the page 2. There should be a hanging indent on every reference. In MS Word, create a hanging indent by going to â€Å"Format† gt; â€Å"Paragraph† gt; â€Å"Special† gt; select Hanging from the drop down menu. 3. Place references in alphabetical order 4. Reference page should be double-spaced like the rest of the paperno extra spaces| Journal article with a doi (Digital Object Identifier):| Zembylas, M. (2008). Adult learners’ emotions in online learning. Distance Education, 29(1), 71-87. doi: 10. 1080/01587910802004852  | Journal article from a secure database (like our library) with no doi:| Tang, F. , Chou, S. Chiang, H. (2005). Students’ perceptions of effective and ineffective clinical instructors. Journal of Nursing Education, 44(4), 187-192. Retrieved from ProQuest database. | Journal article from the Internet with no doi:| Vesely, P. , Bloom, L. , Sherlock, J. (2007). Key elements of building online community: Comparing faculty and student perceptions. MERLOT Journal of Online Learning and Teaching, 3(3), 234-246. Retrieved from http://jolt. merlot. org/vol3no3/vesely. pdf  | Book| Weimer, M. (2002). Learner-centered teaching: Five key changes to practice. San Francisco: Jossey-Bass. Chapter in a book with multiple authors| Stake, R. E. (2005). Qualitative case studies. In N. K. Denzin Y. S. Lincoln (Eds. ), The Sage handbook of qualitative research (3rd ed. , pp. 443-466). Thousand Oaks, CA: Sage. | Website with an author| Berwick, D. (n. d. ). Don Berwicks challenge: Eliminate restrictions on visiting hours in the intensive care unit. Retrieved from http://www. ihi. org/knowledge/Pages/ImprovementStories /DonBerwicksChallengeEliminateRestrictionsonVisitingHoursinthe IntensiveCareUnit. px | Website without an author| Carnegie Foundation for the Advancement of Teaching. (2009). Assessment for improvement: Tracking student engagement over time. Retrieved from http://nsse. iub. edu/NSSE_2009_Results/pdf/NSSE_AR_2009. pdf  |   Ã‚  Note: In an APA paper all references would be double-spaced Warning: Indicates a common error. Regarding references: Use only scholarly sources for your assignments. What are scholarly sources? * Peer reviewed journals—examples: Journal of Nursing Education, American Journal of Nursing, Journal of Holistic Nursing. Use only articles available in full text (not just an abstract). * Ideally the articles will have been published in the last 5-10 years. * Text books * Reputable websites—examples: http://nursingworld. org/, http://www. cdc. gov/, http://www. ihi. org/Pages/default. aspx * Wiki’s such as Wikipedia are NOT scholarly sources—do not use. File Name: At American Sentinel University, always name your document (what you save as) using this format: YourName_CourseNumber_Assignment This guide is provided as a convenient reference, but always refer to the APA

Amended Budget Paper Essay Example | Topics and Well Written Essays - 750 words

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Book report - Jack Welch - Winning Essay Example | Topics and Well Written Essays - 750 words

Book report - Jack Welch - Winning - Essay Example The first section of the book, Underneath It All, uncovers the essence and reason for the founding and creation of any particular company. A business cannot run without the mission/vision statements but if candidly discussed and shared with every member of the organization, reaping the rewards will become more effective. Welch describes the mission statement as an important tool to set the company values in motion. Without a concrete mission formulated by the upper management, the values which motivate and empower the rest of the hierarchy will be lost (Welch, 2005). Values are the driving force behind the mission and they may not prove to be successful if every employee’s input is not included. This step will only reinforce the mission and hold each member of the company accountable to those values. These tools cannot be used effectively without the beauty of open-mindedness. Welch further suggests that candor is one tool which is never fully used because the more it is used, the more it is needed. Enabling a culture of candor in an organization opens several avenues of communication, ideas and frankness pushing the company into the winning position. After defining the boundaries, the company head needs to manage his group according to the beliefs he has imbibed. With this premise, Welch introduces the second section, Your Company, which discusses the principles of hiring and firing, people and crisis management, change and leadership. Being a first-class leader means following the eight rules of leadership with some examples such as confidence-building for the team, positive energy along with optimism and not to let the position get to your head. Managing Human Resources is what distinguishes the successes of companies. Elevating the HR Manager’s position and importance to that of the company’s CFO is the key to effective people management (Welch, 2005). Using what Welch describes as the 4-E (and 1-P) test for a winning team which

Couch Surfing the website Essay Example | Topics and Well Written Essays - 250 words

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Analyze love or power and control on two sheakesperean plays and Essay

Analyze love or power and control on two sheakesperean plays and feminist literature, and feminist research - Essay Example ian leash, Shakespeare openly created female characters to transcend socially permitted female behaviors of the time and exposed female characters and their manipulations and power games they tried to play. All the heroines of his plays all have a deep trace of rebellion and some feminist views opposing male dominance and control. Seen from a feminist perspective Lady Macbeth was a strong-willed, intelligent woman, who was driven to consorting with devils because the male demons of her own culture prevent her from doing what men assumed by right. Her wish to shed her sexual identity, as seen through the â€Å"unsex me here† line, reinforces desire for power and control in a male dominated world. Similarly Hermia can be seen as a female who despite the penalty of death on disobedience rebels against the control of her father and the King so that she can choose her own life partner. Macbeth: Shakespeare’s Macbeth is a tragedy where both the villains Macbeth and his Lady are also the tragic heroes of the play.   A tragic hero is a character that the audience sympathizes with despite his/her actions that would indicate the contrary.   Macbeth, in spite of his horrible murders, is a pitiable man.   He suffered because he could not enjoy his royal status as  fear, paranoia and sleeplessness plagued him.  Similarly Lady Macbeth is also a tragic hero.   Her early ambition and daring did not last long, and she soon deteriorates into a delusional, hapless somnambulist.   She breaks down due to the strain of the crime.   Macbeth and his wife are pitiable characters because the audience is able to follow their every thought and action and can see the how their greed is followed by guilt and remorse.   A Midsummer Nights Dream: A Midsummer Nights Dream is a comedy in which Shakespeare shows many different kinds of love and marriage with the underlying theme of male dominance and their women’s rebellion against this control. Helena and Hermia flee into the woods on

Case Studies of Organizational Learning in Terrorist Groups Essay

Case Studies of Organizational Learning in Terrorist Groups - Essay Example For example, the terrorist group Al-Qaeda based in the Middle East uses Islam to support their jihad or Holy War, which targets non-Muslims. These terrorist factions use brainwashing tactics to recruit members. Religious extremists are easier to recruit because they already possess fanatic ideas pertaining to religion. Presently, sub-groups emerge in different areas of the world with affiliations to a bigger group. The Al-Shabaab based in Somalia is affiliated to the terror group Al-Qaeda. The function of the herald group is to supply money and ammunitions to the smaller groups, which are crucial for engaging in terrorist activities. This emerging trend makes combating terrorism a harder task for intelligence agencies. Plenty of monetary and human resources are deployed to monitor the different groups. In conclusion, intelligence agencies across the globe face difficulties in trying to mitigate terrorism molded from stringent religious ideologies. This is because it is impossible to reason with religious extremists who believe their actions are sanctified by a

Couch Surfing the website Essay Example | Topics and Well Written Essays - 250 words

Couch Surfing the website - Essay Example The vision of the website is to see a world where everyone can explore and create friendships and connections with anyone that they encounter. The site has a rough estimate of about ten million users in more than two hundred different countries around the world. The site can be useful for students in student exchange programs and hitchhiking communities. Students and journalists use the site to meet new people, to hear about their travels, and make long lasting friendships. For professional journalists, this site can be of great use to them to learn about new places that they can visit. Website owners can use this website to create awareness for their websites. This is easy because one can generate traffic to their own website by connecting with friends on the social network site and directing them to website by providing web links. The platform is also useful for discussion forums where you can post pressing issues and invite friends to discuss them. This way, a student can get ideas about a project that needs assistance from the website

Case Studies of Organizational Learning in Terrorist Groups Essay

Case Studies of Organizational Learning in Terrorist Groups - Essay Example For example, the terrorist group Al-Qaeda based in the Middle East uses Islam to support their jihad or Holy War, which targets non-Muslims. These terrorist factions use brainwashing tactics to recruit members. Religious extremists are easier to recruit because they already possess fanatic ideas pertaining to religion. Presently, sub-groups emerge in different areas of the world with affiliations to a bigger group. The Al-Shabaab based in Somalia is affiliated to the terror group Al-Qaeda. The function of the herald group is to supply money and ammunitions to the smaller groups, which are crucial for engaging in terrorist activities. This emerging trend makes combating terrorism a harder task for intelligence agencies. Plenty of monetary and human resources are deployed to monitor the different groups. In conclusion, intelligence agencies across the globe face difficulties in trying to mitigate terrorism molded from stringent religious ideologies. This is because it is impossible to reason with religious extremists who believe their actions are sanctified by a

Prader-Willi Case Essay Example for Free

Prader-Willi Case Essay This essay will discuss the role of the nurse in the context of Prader-Willi syndrome (PWS) which is caused by a genetic disease by a deletion in chromosome 15. This can lead to insatiable hunger, excessive eating and result in obesity. This syndrome requires management from the multidisciplinary team which includes dieticians, doctors, mental health team, nurses, occupational therapist, physiotherapists and social services. This is where the role of the nurse and nursing staff can stand out as they are actively involved in patient care providing support to the patient and their family, as well as playing a role in preventing disease progression. â€Å"Make the care of people your first concern, treating them as individuals and respecting their dignity† (NMC, 2010). This is of utmost importance in the management of PWS, and how the individual can be educated by managing the syndrome. This essay will relate across the lifespan; childhood, adolescence and adulthood. This will be discussed in the following paragraphs. PWS is an uncommon genetic disorder that is present at birth in either male or female. It is the most common genetic cause of morbid obesity and can vary at different weights. Although the cause is complex, it results from a deletion or unexpression of genes from the paternal chromosome 15. This condition affects approximately 1 in 10,000 to 1 in 25,000 new-borns (Killeen, 2004). Individuals with this condition have serious problems controlling their weight as they have a very strong food compulsion before the age of six. The condition is diagnosed through genetic testing. It is specifically DNA-based methylation testing to distinguish the absence of the paternal chromosome; chromosome 15.This test is recommended for new borns with pronounced hypotonia (praderwillisyndrome, 2010). An early diagnosis allows for early intervention as well as early provision of growth hormone (GH) treatment. GH gives an increased muscle mass and supports linear growth. GH treatment also advantageous because it decreases food preoccupation and weight gain. During pregnancy, there can be a few abnormal signs which can indicate, but are not limited to PWS. In utero, there can be excessive amniotic fluid; a condition known as polyhydraminos. There can also be reduced fetal movements and the fetal position within the uterus may be suboptimal i.e. breech presentation. Once the baby is born, other signs such as feeding difficulties- due to poor muscular tone affecting the sucking reflex and generalised hypotonia-poor muscular tone (FPWR, 2011).The baby may feel floppy when held as their joints may be loosely extended instead of being firmly in position. An early diagnosis of these can point to an early diagnosis of PWS, hence lead to early management. The clinical presentation of PWS is not limited to physical signs and symptoms but includes linear growth and development, which can cause mental and behavioural problems. These can be presented early in childhood. Physical features can include short stature, small hands and feet, low birth weight, and classic facial features including narrow forehead, almond-shaped eyes and â€Å"down-turned† mouth (Holm et al, 1993). Behavioural symptoms can include obsessive behaviours, unpredictable temper tantrums, skin picking, stubbornness and resistance to change. Individuals with this condition are not mentally stable as they have an increased risk and suffer from depression and psychosis. They also suffer from hallucinations, loss of interests, changes in mood and poor concentration levels. As mentioned earlier, Hypotonia is poor muscle tone. Hypotonia improves with age, however if it persists by the age of two to three, it is very likely that the child may not have started walking. Walking is a crucial milestone that should be reached within the first two years of life (NLM 2010).This is because their weight gain has made it difficult to move around and their condition is already exacerbated by the hypotonia. They can be referred to physiotherapy to try and improve the muscle tone. They also have a failure to thrive and their rate of physical growth is less than their peers’. With failure to thrive, these infants may not respond to simulation as they tire easily. Infants with this condition gain weight more slowly and start to put on more weight by the age of 2-3. A child with PWS may start speaking later than other children as their verbal skills are delayed. Speech and language therapy is advisable at this point as the child will benefit with input from a ther apist. Most common speech concerns include problems with voice quality, articulation, usage as well as resonance patterns (Munson-Davis, 1988). The child constantly craves for food and eats more than they should. They constantly gain weight and may eat things most people wouldn’t deem edible; such as expired or frozen food. There is a serious compulsion towards food, and a lack of awareness of hunger satiation. In childhood, they have a tendency to be stubborn, argumentative and possessive (Nordqvist, 2010). Some infants can develop obsessive compulsive disorder (OCD) along with repetitive behaviours. They can throw tantrums as they can only consume a certain amount of food per day so they do not gain weight (as per their dietary management plan). During adolescence, height becomes more noticeable as the individual is much shorter than others. The height of a female with PWS on average is 4 feet 10 inches while that a male with PWS is 5 feet 2 inches (nhs.uk, 2011). The individual would still suffer from hypotonia up until adulthood and would be extremely flexible due to poor muscle tone. Once the individual has reached adulthood, they cannot reproduce as they are infertile due to delayed puberty in both male and female from a young age. The reproductive system would not have produced enough sex hormones, which results in undeveloped sex organs. Hypogonadism is a medical term for the reduction or absence of hormone secretion or other physiological activity of the gonards. Individuals with PWS have some degree of a learning disability. Learning disability nursing practice reflected current philosophies of supporting people with learning disabilities (Clifton et al. 1992). The presence of PWS in a family can create substantial stress. Families would have had to adapt to changes within the household to be able to manage the individual with the syndrome. Parents are often exhausted from the demands of their time and energy for diet control, specialized programmes, therapy appointments and behavioural supervision. Siblings are also affected as they often feel neglected as the PWS sibling receives more attention and appears to be more loved. (Tomase-ski-Heinemann 1998) It is a nurse’s responsibility to help support and manage a patient with the condition along with supporting the individual and their family. The uncontrollable appetite leads to obesity. Obesity is a global epidemic, and is also known to be a significant risk factor for other health related problems which include heart disease, diabetes, high cholesterol, hypertension, hypoventilation and right sided heart failure (WHO, 1948). Some people with PWS also develop type 2 diabetes mellitus which is the most common form of diabetes, where the body either does not produce enough insulin or the insulin is not working properly; insulin resistance. In addition, part of the due diligence of nurses is that in providing care for a patient with PWS that they holistically look after the patient starting from the first interaction. For example, in PWS this involves first building a rapport with the patient explaining their diagnosis and describing the nurses’ role in the management (monitoring weight, supporting diet). It should be stressed to the patient and their family the importance of confidentially and that their information will not be told to anyone outside the medical team. This ensures that the nurse has fulfilled their responsibly within the multi-disciplinary team. The Data Protection Act (1998) was put in place to maintain patient records and information. Therefore the nurse would be upholding these legislations by practising patient confidentiality. When visiting a patient at home or in the hospital, a nurse should ask for consent for patient contact i.e. assessing vital signs. Nurses’ must follow the NMC guidelines although the patient may not understand what the nurse is saying because of a learning disability for example. However, consent must be indicated in some form such as nodding of the head. The NMC (2010) states that â€Å"you should ensure that you gain their consent before you begin to provide care†. If the patient is unable to give consent and is alert, the next of kin is assigned to making the decision due to the best interest of the patient. A nurse is accountable to manage, maintain and monitor the individuals’ weight. The nurse does not only have to keep track but also the family should be involved in managing the weight. Nurses can book weekly appointments with the individual and their family/carer so their weight can be monitored to check for any improvement to the weight or not. The family should monitor the amount the individual consumes daily. They are constantly hungry and cry for more food if it is not given to them. Locks must be placed on cupboards or on the kitchen door to stop them from eating (PWSAUSA, 2009). It will be hard for the individual to cope once this is introduced as they do not know when to stop. This is where the nurse should explain to the individual how important it is to manage their weight and what it can lead to if it is not controlled. The nurse should be there to support them when the individual starts to show aggressive behaviour as it will be hard for the family as well. Adults with PWS are inactive due to their low muscle tone and therefore only require 1,000-1,200 calories a day (PWSA, 2010). Encouraging the individual to be healthy is important. Although the nurse must understand that the patient may be unable to exercise properly due to poor muscle tone, they should encourage the patient to eat healthily for example fruit and vegetables. The individual must not have too many fatty foods i.e sweets and chocolate. By promoting healthy foods will ensure that the individual does not gain more weight than they should. It will be hard for the individual to cope with the new foods introduced to them which is why a nurse will be there to support the individual and family. The nurse can also advise the family on encouraging the patient to do some exercises i.e. helping with house chores. Any sorts of movement can help burn calories. Communication skills is one of the key skills a nurse should have. â€Å"To understand the process of communication, we must understand how people relate to each other† (Faulkner, 1982). Supporting and helping patients and their families, communication is crucial. By managing the individual and their condition, team work is fundamental. It is important to work as team as the main focus in the patient care plan is the individual. Each health care professional has a role to play to help improve the individuals well-being. The main focus is the role of the nurse and how their professional issues can impact on the health and illness of people across the life span. It is important for a nurse to understand individuals and their condition because they can help make it somewhat easier for the patient and their family. This is because the nurse is an allied health professional who enjoys more interaction with the patient than many other members of the multidisciplinary team. Nurses need to respect patients from various backgrounds as PWS can affect people of all ethnicities. This syndrome can be found in people of any ethnic background (Zelweger, 1983). Nurses’ must respect the patients’ background and understand that they may not be able to communicate or understand what is being said. Makaton could be used to enhance communication and is a language programme, which is designed to provide a means of communication to individuals who cannot communicate well by speaking (Beukelman. D.R Mirenda). Makaton can also be used with individuals who have cognitive impairments and specific language impairment that have negatively affected the ability to communicate. An interpreter is also a form of communication as they are translating what the other is saying if English is not their first language. By using interpreters (sign language or foreign languages) will help the patient and the nurse understand what the other is saying i.e. explaining what the condition is. This will also leave the patient happy so they do not feel angry and upset. Some individuals may have a language barrier or cultural beliefs which can go against some forms of treatment. The syndrome is lifelong and unfortunately has no cure, but with the support and advice the nurse will have given the patient and the family, the patient will be happy and content (FPWR, 2010). This essay has included the role of the nurse for this condition and how it can be managed. Overall, the main point is to promote a healthy way of what the individual eats and how it can be managed. Keeping such foods out of sight and having a positive family, helping the individual through the tough times can promote a healthy way of living for the individual. Exercise is crucial in maintaining a healthy weight.